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  • Colilert-18 water testing product information
    million heterotrophs per 100 ml and 5 million per 250 ml Eliminates the subjective interpretation found in traditional methods Detects a single viable coliform or E coli per sample Economical Minimises evening and weekend work Up to 15 month shelf life at room temperature Flexible Can be used for presence absence P A or quantification testing with Quanti Tray and Quanti Tray 2000 Resource List Colilert 18 Procedure Australian Standard Approval for Colilert Colilert 18 Quanti Tray and Quanti Tray 2000 Colilert 18 Quanti Tray Comparison with EU Drinking Water Directive Reference Method How Colilert 18 works Colilert 18 uses Defined Substrate Technology DST nutrient indicators ONPG and MUG to detect coliforms and E coli Coliforms use their β galactosidase enzyme to metabolise ONPG and change it from colourless to yellow E coli use β glucuronidase to metabolise MUG and create fluorescence The few non coliforms that do have these enzymes are selectively suppressed by Colilert s specifically formulated matrix This approach is different from traditional media which provide a nutrient rich environment that supports the growth of both target organisms and non targets When non targets grow and mimic target organisms false positives occur Growth of non targets can also suppress target organisms and give false negatives in traditional media To suppress non targets traditional media often include high levels of salts detergents or other selective agents that may inadvertently suppress target organisms and give further false negatives All IDEXX DST reagents can be used in a presence absence P A vessel Quanti Tray or Quanti Tray 2000 Only IDEXX reagents can be used with Quanti Tray and Quanti Tray 2000 View Print Colilert 18 Procedure Learn how to use Colilert 18 Learn how to use Quanti Tray Learn how to use Quanti Tray 2000 A step by step guide to using Colilert 18 Show detail Hide detail Presence Absence P A and Quantification Instruction Presence Absence P A Quantification Step 1 Add reagent to sample and incubate at 35 0 5 C for 18 hours Step 1 Add reagent to sample Step 2 Read results Colourless negative Yellow total coliforms Yellow fluorescent E coli Step 2 Pour into Quanti Tray counts from 1 200 or Quanti Tray 2000 counts from 1 2 419 Step 3 Seal in Quanti Tray Sealer and place in 35 0 5 C incubator for 18 hours in other countries the temperature requirement may be different per regulatory requirements View the faecal coliform procedure Step 4 Quanti Tray Read results Yellow wells total coliforms Yellow fluorescent wells E coli Quanti Tray 2000 Read results Yellow wells total coliforms Yellow fluorescent wells E coli Frequently asked questions and answers Is Colilert 18 an ISO method Yes The International Organization for Standardization ISO has withdrawn ISO 9308 2 1990 which follows the multiple tube most probable number method and replaced it with IDEXX Colilert 18 Quanti Tray the new worldwide ISO standard 9308 2 2012 for detecting total coliforms and E coli in water Learn more

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/colilert-18.html (2014-01-05)
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  • Colisure water testing product information
    coliforms and E coli simultaneously in 24 hours or less No confirmations needed No glassware cleaning or colony counting Accurate Definitive results at 24 hours can be read up to 48 hours Identifies E coli specifically eliminating unneeded public notification due to non target organisms Suppresses up to 2 million heterotrophs per 100 ml Eliminates the subjective interpretation found in traditional methods Detects a single viable coliform or E coli per sample Economical Minimises evening and weekend work Up to 12 month shelf life at room temperature Flexible A Colisure snap pack can be used for presence absence P A or quantification testing Quanti Tray provides counts to 200 100 ml without dilutions Quanti Tray 2000 provides counts to 2 419 100 ml without dilutions Resource List Colisure Procedure Product Information Sample size 100 ml Time to results 24 hours with a 48 hour read window Shelf life Up to 12 months at 2 25 C Read Sample turns magenta when coliforms are present and fluoresces to indicate the presence of E coli Uses Presence absence or quantification using IDEXX Quanti Tray Quanti Tray 2000 Snap packs for 100 ml water samples 20 test pack No WCLS20I 100 test pack No WCLS100I 200 test pack No WCLS200I How Colisure works Colisure uses Defined Substrate Technology DST nutrient indicators CPRG and MUG to detect total coliforms and E coli Coliforms use their β galactosidase enzyme to metabolise CPRG and change it from yellow to magenta E coli use β glucuronidase to metabolise MUG and create fluorescence View Print Colisure Procedure Learn how to use Colisure Learn how to use Quanti Tray Learn how to use Quanti Tray 2000 A step by step guide to using Colisure Show detail Hide detail Presence Absence P A and Quantification Instruction Presence Absence P A Quantification Step 1 Add reagent to sample and incubate at 35 0 5 C for 24 hours Step 1 Add reagent to sample Step 2 Read results Yellow negative Magenta total coliforms Magenta fluorescent E coli Step 2 Pour into Quanti Tray counts from 1 200 or Quanti Tray 2000 counts from 1 2 419 Step 3 Seal in Quanti Tray Sealer and place in incubator at 35 0 5 C for 24 hours Step 4 Quanti Tray Read results Magenta wells total coliforms Magenta fluorescent wells E coli Count positive wells and refer to MPN table Quanti Tray 2000 Read results Magenta wells total coliforms Magenta fluorescent wells E coli Count positive wells and refer to MPN table Frequently asked questions and answers Is Colisure approved by the US EPA Yes Colisure is US Environmental Protection Agency EPA approved as a method for total coliforms and E coli detection in drinking water and for E coli detection in groundwater What is the detection limit of Colisure Colisure detects coliforms and E coli at 1 organism 100 mL What is the shelf life of Colisure Colisure shelf life is up to 12 months from date of manufacture What are the

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  • Enterolert water testing product information
    Science How To Q A Accessories Resources Enterolert Be confident in your outcomes Easy No media preparation No autoclaving No colony counting No glassware cleaning Rapid Less than 1 minute of hands on time Results in 24 hours rather than 48 to 72 hours Accurate Sensitive to 1 enterococci 100 ml Enumerates up to 2 419 enterococci per 100 ml without dilutions with Quanti Tray 2000 Less subjective interpretation 50 fewer false positives and 95 fewer false negatives than the standard membrane filtration MF method 1 Economical Up to 12 month shelf life minimises waste 24 hour test saves incubator space 1 Budnick GE Howard RT and Mayo DR Evaluation of Enterolert for Enumeration of Enterococci in Recreational Water Applied and Environmental Microbiology 1996 62 3881 3884 Resource List Enterolert Procedure Product Information Sample size 100 ml Time to results 24 hours Shelf life Up to 12 months at 2 30 C Read Sample fluoresces to indicate the presence of enterococci Uses Presence absence or quantification using IDEXX Quanti Tray Quanti Tray 2000 or multiple tube Most Probable Number MPN testing Snap packs for 100 ml water samples 20 test pack No WENT020 100 test pack No WENT100 200 test pack No WENT200 How Enterolert works Enterolert uses a Defined Substrate Technology DST nutrient indicator to detect enterococci This nutrient indicator fluoresces when metabolised by enterococci DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media View Print Enterolert Procedure Learn how to use Enterolert Learn how to use Quanti Tray Learn how to use Quanti Tray 2000 A step by step guide to using Enterolert Show detail Hide detail Quantification Instruction Quantification Step 1 Add reagent to sample Step 2 Pour into Quanti Tray counts from 1 200 or Quanti Tray 2000 counts

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/enterolert.html (2014-01-05)
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  • Enterolert 250 water testing product information
    Resources Enterolert 250 A convenient format for confident outcomes Easy Ready to use media Simple procedure Unit dosed packaging eliminates media preparation Reagent contains no toxic compounds Rapid Confirmed results in 24 hours Simplified quality control Less than 1 minute of hands on time Accurate More accurate when compared to ISO 7899 2 on all samples Liquid enrichment ensures optimised recovery Eliminates subjective interpretation of results determined by traditional methods Resource List Enterolert 250 Procedure Product Information Sample size 250 ml Time to results 24 hours Shelf life Up to 12 months at 2 25 C away from direct light and humidity Read Any change from the original colour to green is considered a positive result No ultraviolet light source is required Uses Presence absence testing Snap packs for 250 ml water samples 20 test pack No WENT250 20 100 test pack No WENT250 100 How Enterolert 250 works Enterolert 250 uses a Defined Substrate Technology DST nutrient indicator to detect enterococci Enterolert 250 provides confirmed presence absence results in 24 hours Enterolert 250 utilises orth nitrophenyl ß D glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation When the substrate is metabolised by enterococci the sample turns from blue to green to indicate detection Any change from the original colour to green is considered a positive result No ultraviolet light source is required Enterolert 250 detects enterococci at 1 cfu in 250 ml bottled water samples in 24 hours View Print Enterolert 250 Procedure Learn how to use Enterolert A two step guide to using Enterolert 250 Show detail Hide detail Presence Absence P A Instruction Presence Absence P A Step 1 Add reagent to sample shake to mix and then incubate at 41 1 C for 24 hours results are valid for up to 28 hours of incubation Step 2 Blue indicates negative for enterococci Green indicates positive for enterococci Frequently asked questions and answers How does the product work Enterolert 250 is designed to detect Enterococcus species in bottled water samples It is based on IDEXX s patented Defined Substrate Technology DST Enterolert 250 utilizes ortho nitrophenyl ß D glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation When the enzyme substrate is metabolized by the enterococci the sample turns from blue to green to indicate a positive detection Any change from the original color to green is considered a positive result No ultraviolet light source is required Enterolert 250 detects enterococci at 1 cfu in 250 mL bottled water samples in 24 hours What species of enterococci can Enterolert 250 detect Enterolert 250 is designed to detect enterococci in bottled water As with any microbial method some Enterococcus species are more easily detected than others Some examples of Enterococcus species we have detected in trials and studies Enterococcus faecalis Enterococcus faecium Enterococcus hirae Enterococcus gallinarum Enterococcus casseliflavus Enterococcus durans Enterococcus avium Is there a comparator for Enterolert 250 There is no comparator

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/enterolert-250.html (2014-01-05)
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  • Enterolert-DW water testing product information
    Enterolert DW Quanti Tray Method for Determination of Enterococci in Drinking Waters Enterolert DW Quanti Tray Method Included in the United Kingdom Blue Book Product Information Sample size 100 ml Time to results 24 hours Shelf life Up to 12 months at 2 25 C away from direct light and humidity Read Any change from the original colour to green is considered a positive result No ultraviolet light source is required Uses Quantification testing using IDEXX Quanti Tray Quanti Tray 2000 Snap packs for 100 ml water samples 20 test pack No WENTDW 020 100 test pack No WENTDW 100 200 test pack No WENTDW 200 How Enterolert DW works Enterolert DW uses a Defined Substrate Technology DST nutrient indicator to detect enterococci When coupled with the IDEXX Quanti Tray System Enterolert DW provides quantitative confirmed results in 24 hours Enterolert DW utilises ortho nitrophenyl β D glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation When the enzyme substrate is metabolised by enterococci the sample turns from blue to green to indicate detection Any change from the original colour to green is considered a positive result No ultraviolet light source is required View Print Enterolert DW Procedure Learn how to use Enterolert Learn how to use Quanti Tray Learn how to use Quanti Tray 2000 A step by step guide to using Enterolert DW Show detail Hide detail Quantification Instruction Quantification Step 1 Add reagent to sample Step 2 Cap vessel and shake to mix Step 3 Pour into Quanti Tray counts from 1 200 or Quanti Tray 2000 counts from 1 2 419 Step 4 Seal in Quanti Tray Sealer and place in 41 0 5 C incubator for 24 hours in other countries the temperature requirement may be different per regulatory requirements Step 5 Count positive wells and refer to Most Probable Number MPN table Frequently asked questions and answers How does the product work Enterolert DW is designed to detect Enterococcus species in drinking water samples It is based on IDEXX s patented Defined Substrate Technology DST Enterolert DW utilizes ortho nitrophenyl ß D glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation When the enzyme substrate is metabolized by the enterococci the sample turns from blue to green to indicate a positive detection Any change from the original color to green is considered a positive result No ultraviolet light source is required What species of enterococci can Enterolert DW detect Enterolert DW is designed to detect enterococci in drinking water As with any microbial method some Enterococcus species are more easily detected than others Some examples of Enterococcus species we have detected in trials and studies Enterococcus faecalis Enterococcus faecium Enterococcus hirae Enterococcus gallinarum Enterococcus casseliflavus Enterococcus durans Enterococcus avium Is there a comparator for Enterolert DW There is no comparator for Enterolert DW For comparison a negative control can be used when interpreting results What is the incubation time and

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/enterolert-dw.html (2014-01-05)
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  • Enterolert-E water testing product information
    enterococci test is right for you View a comparison Questions Call 1300 4 IDEXX or email water idexx com Overview Science How To Q A Accessories Resources Enterolert E Be confident in your outcomes Easy No media preparation No autoclaving No colony counting No glassware cleaning Rapid Less than 1 minute of hands on time Complete results in 24 hours Accurate Correlates with ISO Method 7899 1 Sensitive to 1 enterococci 100 ml Enumerates up to 2 419 enterococci per 100 ml without dilutions with Quanti Tray 2000 Eliminates the subjective interpretation found in traditional methods Cost Effective Up to 12 month shelf life minimises waste 24 hour test saves incubator space Resource List Enterolert E Procedure Product Information Sample size 100 ml Time to results 24 hours Shelf life Up to 12 months at 2 30 C Read Sample fluoresces to indicate the presence of enterococci Uses Presence absence or quantification testing using IDEXX Quanti Tray Quanti Tray 2000 or multiple tube Most Probable Number MPN testing Snap packs for 100 ml water samples 20 test pack No WENTE020 100 test pack No WENTE100 200 test pack No WENTE200 How Enterolert E works Enterolert E uses a Defined Substrate Technology DST nutrient indicator to detect enterococci This nutrient indicator fluoresces when metabolised by enterococci DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media View Print Enterolert E Procedure Learn how to use Enterolert Learn how to use Quanti Tray Learn how to use Quanti Tray 2000 A step by step guide to using Enterolert E Show detail Hide detail Quantification Instruction Quantification Step 1 Add reagent to sample Step 2 Pour into Quanti Tray counts from 1 200 or Quanti Tray 2000 counts from 1 2 419 Step 3 Seal using Quanti

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/enterolert-e.html (2014-01-05)
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  • Pseudalert 250 water testing product information
    than 1 minute of hands on time for streamlined testing work flow Accurate Specifically detects Pseudomonas aeruginosa reducing the risk of false positives from non target organisms Results are definitive without additional confirmation steps Simple fluorescent colour change minimises subjective result interpretation Liquid enrichment optimises recovery Resource List Pseudalert Procedure Pseudalert Quick Reference Guide Product Information Sample size 250 ml Time to results 24 hours Shelf life Up to 12 months at 2 C 30 C store away from direct light Read Sample fluoresces to indicate the presence of Pseudomonas aeruginosa Uses Presence absence Snap packs for 250 ml water samples 20 test pack No WPSE25020I 100 test pack No WPSE250100I How Pseudalert 250 works The Pseudalert 250 test detects the presence of Pseudomonas aeruginosa in bottled water samples The test is based on a bacterial enzyme detection technology that signals the presence of P aeruginosa through the hydrolysis of a substrate in the Pseudalert 250 reagent P aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids vitamins and other nutrients present in the Pseudalert 250 reagent Actively growing strains of P aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light Pseudalert 250 detects P aeruginosa at 1 cfu 250 ml samples within 24 hours for non carbonated water samples and within 26 hours for carbonated water samples View Print Pseudalert 250 Procedure Learn how to use Pseudalert and Pseudalert 250 A two step guide to using Pseudalert 250 Note If using carbonated water please refer to the package insert PDF for sample preparation instructions Show detail Hide detail Presence Absence P A Instruction Presence Absence P A Step 1 Add reagent to sample sample will turn yellow and then incubate at 38 0 5 C for 24 28 hours Step 2 Read under ultraviolet UV light Blue fluorescence indicates presence of P aeruginosa See the Pseudalert Quick Reference Guide PDF for tips on reading fluorescence Frequently asked questions and answers What water types can be tested with the Pseudalert 250 test The Pseudalert 250 test is designed specifically for bottled water samples Bottled water samples are defined as samples taken from any point in the manufacturing of bottled water from the original water to the finished product Pseudalert 250 was specifically formulated and tested to ensure it would detect stressed P aeruginosa in these samples How does Pseudalert 250 work The Pseudalert 250 test is based on a bacterial enzyme detection method that signals the presence of Pseudomonas aeruginosa through the hydrolysis of an enzyme substrate P aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids vitamins and other nutrients present in the Pseudalert reagent Actively growing strains of P aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light Pseudalert 250 detects P aeruginosa at 1 cfu per 250 mL sample within 24 hours What is the detection limit of Pseudalert 250 Pseudalert

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/pseudalert-250.html (2014-01-05)
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  • Invitrogen products, Filta-Max and Filta-Max xpress
    Technologies line of advanced tools for microbiology research complements the IDEXX portfolio of trusted water testing products for Cryptosporidium and Giardia Questions Call 1300 4 IDEXX or email water idexx com Dynabeads Semi automated IMS Manual IMS Filta Max xpress and Dynabeads let you easily and reliably isolate specific biological entities from complex matrices Versatile Dynabeads immunomagnetic separation IMS technology Targets common contaminants present in water food and environmental samples Is widely accepted and routinely used in environmental microbiology laboratories around the world Dynabeads IMS Products Dynabeads are coated with affinity purified antibodies against specific surface markers on microbial pathogens which are common contaminants of food water and environmental samples Dynabeads Products Item Quantity Catalogue Number Dynabeads Cryptosporidium Giardia Combo Kit 1 ml 10 tests 5 ml 50 tests 73002 73012 Dynabeads Cryptosporidium Kit 1 ml 10 tests 5 ml 50 tests 73001 73011 Invitrogen MX7 Mixer Semi automated Immunomagnetic Separation IMS The Invitrogen MX7 Mixer US EPA approved for Method 1623 PDF 92 KB automates the steps of the Cryptosporidium and Giardia magnetised mixing isolation protocols creating a semi automated IMS method The only protocol change from your existing method is the use of a rotator instead of manual rocking making it easy to incorporate the Invitrogen MX7 Mixer into your workflow The same magnetic separators are used but the MX7 Magnetic Mixer significantly reduces the risk of repetitive strain injuries In parallel studies performed by independent laboratories data produced using the MX7 Mixer were equivalent to those obtained following the standard IMS method Semi automated IMS Products Item Quantity Catalogue Number Dynabeads Cryptosporidium Giardia Combo Kit 1 ml 10 tests 5 ml 50 tests 73002 73012 Dynabeads Cryptosporidium Kit 1 ml 10 tests 5 ml 50 tests 73001 73011 IVGN MX1 Mixer 1 unit 15907 IVGN L10 Tubes

    Original URL path: http://www.idexx.com.au/html/en_au/water/products/invitrogen.html (2014-01-05)
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